Friday, April 24, 2015

Reflection: Journal Post 14

This is the final post of the semester and I can say that I am very pleased with my work throughout the semester. I feel that I've become more comfortable with the topic of cell and molecular biology but also know that there is a world of information that continues to grow. Overall, the topic was quite difficult due to the millions of functions and parts to a cell.
With that being said, the class allowed me to appreciate the human body so much more. We wake up in the morning and prepare for our days and try to endure whatever it throws at us. We go to sleep at night feeling exhausted and often times glad the day is over. We stay up for roughly 16 hours in a day and feel like life can be too much sometimes. What we don't realize is that while we are factories filled with infinite workers who do not rest. They work and work and do their jobs wonderfully in order for us to wake up and do what the day needs of us. Its incredible that while we are a single being, we are made up and filled with cells that have purpose and function of their own. As I type this, new cells are regenerating and signals are being sent all throughout my body. Careful cells from my brain send signals through pathways that make it my fingers, which made it possible to type this very sentence.
Taking this class has allowed me to appreciate the hard work our Creator has done. He was so precise and meticulous in his work and it is mirrored in the very cells inside of us. I have become so much more appreciative of what I am and what I am made of--literally.

Dictionary: Journal Post 12

Cyclin D
Its synthesis is stimulated through growth factors through the Ras/Raf/MEK/ERK pathway, which continues to do so as long as growth factors are present.
If cyclin D is continuously expressed, an important regulator of the cell cycle is lost. This mutation can lead to conditions such as breast cancers and lymphomas.
Cyclin D is an oncogene that drives cell proliferation as well as targets Rb, a cancer supressor gene.




Cell Cycle Checkpoints
Spindle assembly checkpoints stops mitosis at metaphase if chromosomes are not properly aligned.
DNA damage checkpoints ensure that damaged DNA is not replicated and passed off to daughter cells.



Encounter: Journal Post 11

It has come to my attention that I spend WAY too much time online. I scroll through so many pages and find myself looking at topics that are so farfetched and unrelated to the work I was doing. Occasionally, I'll find myself doing something related school--miraculously--and find topics related to cell bio that interest me.

Recently, I came across a short article that spoke about functional differences in cells that have similar genotypes. I already knew that cells have multiple functions and perform in the roles they need to when the environment is correct. It is possible for cells with similar genotypes to behave in different ways. Because this is something that can occur with cells, it makes research on cells more difficult when it comes to studying them. This is especially true for cancer research when studying tumors and the way they behave within the body. 

I feel that in knowing this information, I have an idea of how prepared I need to be for my career. I plan to become a surgeon so dealing with tumors and cancerous cells may be something that will happen frequently. One must always understand what their dealing with and be prepared for whatever situation comes up. It is important to know how the "enemy" behaves because it allows me to be ready for whatever approach I need to take.

Friday, April 17, 2015

Investigations: Journal Post 13

In the past few weeks, I have been preparing for my project which will be presented on Monday during the convention. The subject I chose was depression, a topic that is very close to me. I have suffered from depression for a long time so it only seemed natural to talk about something that I could speak about from experience.

In my studies, I focused on the monoamine hypothesis of depression. This theory states that one cause for depression may be an issue with neurotransmitters and their receptors in nerve cells. The idea is that there are perhaps too few neurotransmitters causing a lack of stimulus with receptors.
This research got me thinking about how the correlation of maltreatment and abuse at a young age could heighten low neurotransmitter levels to something more negative. In my research, I found that while it is not a certainty, there may very well be a link to monoamine deficiency and childhood trauma which heightens the symptoms of depression.

One interesting point I found combined genetics and cellular pathways into one possible cause for depression. The monoamine oxidase A gene of MAO-A gene is an enzyme that degrades the level of certain neurotransmitters such as serotonin and norepinephrine. When functioning normally, levels of neurotransmitters are stable and a healthy reaction with receptors in nerve cells occurs. When there is a mutation in the gene, it does not function correctly and exaggerates symptoms of depression, which is similar to the monoamine hypothesis. A study conducted showed that individuals with the MAO-A gene mutation and were exposed to childhood trauma grew up to be hostile, problematic adults. When not treated with care, symptoms can become heightened, making it very difficult for someone to cope and function normally.

If a child is effected by this mutation, they are unable to be emotionally stable and experience confusing changes in mood. They feel lethargic and anxious. When also exposed to harsh childhood experiences, these emotions and symptoms are taken to unbelievable levels.

Another study showed that some serial killers had this mutation of the gene alongside experiences of childhood trauma.
Again, the reasons behind depression are not a certainty and there is not one reason as to why depression occurs. Everyone is unique and reacts differently do it.At a young, tender age, abuse is more influential than ever and can lead to adulthood depression that can be worse than that of someone who had not been abused.

It is interesting knowing how much everything in the body is directly affected by the things around it. Childhood abuse is no light matter and it is obvious it will lead to trauma later on in life. Unfortunately, some do not understand the dynamic of the situation and don't understand just how impactful it is.


For more information, click links above.


Friday, March 27, 2015

Reflection: Journal Post 10

The semester is winding down! These points in the semester usually feel like a weight is slowly but surely being lifted off my shoulders. This year, however, feels different. I can feel the world pushing down on me as a reminder that my college years are coming to a close.

Taking Cell and Molecular Biology has opened my eyes to a completely different side of biology. A cell has so many functions and contains small individual pieces within it that also have many functions. I seek a profession in medicine as a surgeon and want to be the best surgeon I can be. I want to make the most of my education and while I may slack off sometimes, my intentions are good and my mind is in the right place. When I first started the class, the concept was so specific and felt that I wouldn't like the class because of this. It wasn't until recently--unfortunately--that I became more focused and determined in this class.

My biggest realization these past couple weeks has been that the path I've chosen is no cake walk. Of course, I already knew that but I realized the depth of the entire image. There are so many little pieces that could affect the way I treat a patient as a medical professional. The same dedication and focus I put into my work now has to remain constant for the rest of my studies and career as a medical professional. 

I don't know how this class will end for me (even though I'm hoping for a decent grade.) I do know that this is the time for my determination and hard work to kick it up a few notches. I'm ready to love what I do and give it my all.


Friday, March 20, 2015

Dictionary: Journal Post 9

The following terms are from Chapter 12 which deals with the cytoskeleton and cellular movement. I chose these terms because I wanted to have a better understanding of their structure and roles in the cell.


Actin is a protein that forms microfilaments. It is also a main component in the structure of the cytoskeleton. One particular function it has in cellular movement is in muscular contraction where it interacts with filaments of myosin, another protein used in muscle contraction. Actin filaments are typically small, thin filaments and contain polarity. The monomers in actin are added at a rate that is proportional to concentration. This is characterized by the term critical concentration where the rate of polymerization equals the rate of dissociation. Actin can be organized in multiple ways, which include bundled or network organization.








Intermediate Filaments are the second of the three cytoskeletal filaments. They help provide mechanical support for the plasma membrane as well as function with other filaments in the cytoskeleton. They provide tension and resist stretching which keeps the shape of the cytoskeleton. These filaments do not contain polarity. Vimetin and Desmin are some intermediate filaments which help to form the cytoskeleton.





Microtubules are a filamentous structure which are important for their role in cellular movement. They also play a role in cellular division, the cytoskeleton, and intracellular transport. Microtubules are formed by tubulin. Tubulin heterdimers assemble into protofilaments and then to microtubules. Microtubules also place organelles in the cytoplasm.









For more information, the following websites can be visited.

Microtubules
Actin
Intermediate Filaments


Friday, March 6, 2015

Encounter: Journal Post 8

While finding a topic for my paper this week, I found myself searching through many different news articles in search of something interesting enough to write about. As I sit by myself in my room with  my computer, I came across an article entitled "Northeastern Researchers Connect Diseases on Their Molecular Similarities." The article spoke of a team of scientists working for Northeastern University who made a mathematical tool that is designed to analyze in the molecular interactions between cells. With this research they found some unusual connections and relationships to between certain diseases.
The team studied almost 300 diseases that have 20 genetic similarities and found that nearly 75 percent of these diseases were related and were located in a specific location of an interactome--a map of molecular interactions between cells. This information and research provides more information on how certain diseases and cells work together. They are able to understand that the interactions and relationships are dependent on their location in the interactome and overall how to approach certain diseases.
As I do more research and as I progress in this class, I am beginning to realize how great the scientific community is and how much it is advancing while many people have no realization of its advancement. Of course we as a society know that we are much smarter and more prepared scientifically and medically to handle certain scenarios than we were a hundred years ago, but I'm not sure everyone understands the magnitude. I learned that I definitely didn't. It is important that I come across articles and knowledge such as this because it will help me be more prepared for my future as a professional.


Friday, February 27, 2015

Investigations: Journal Post 7

After spending hours upon hours staring at a computer screen doing homework, I decided to steer away from my work and engage in aimless internet activity. This probably wasn't a good idea at the time but little did I know I would be lead to a link that would be productive for my blog this week.

I typically go on Tumblr or Pinterest and scroll through pages of nothing. I follow many science related blogs so I'd like to think I get a good daily dosage of educational content. One link lead to another and I came across a recent news article posted yesterday that was rather interesting. The article, which can be accessed here, spoke of a research conducted by Steve Caplan Ph.D. , UNMC researcher,  who was studying cilia and their effects on human development. He discovered that cilia are used in monitoring the environment surrounding the cell as a way to monitor and stabilize signals sent to the cell in order to control tissue development. In some cases, disorders occur where the development of certain cells and features are disturbed. These disorders are known as ciliopathies.

After reading this article, I became interesting in this type of disorder and felt I needed to learn more about it. I was intrigued about how small something can be, such as cilia which are microscopic filaments on a cell, and still cause a world of chaos when something goes wrong with them.

Ciliopathies are disorders characterized as as genetic mutation which encodes for proteins that are defective. This causes abnormal formation and functions of the cilia. These abnormalities in in kidneys, eyes, nervous system and other systems of the body. This disease may also lead to greater deficiencies such as diabetes, skeletal malformation, and liver problems (Waters & Beales, 2011).

Over 20 known ciliopathic diseases are known to this day with 1 in 1000 people effected. The studies that the doctor was conducting will hopefully provide insight on the complete function and interactions which cause disease such as this (Ciliopathy Alliance, 2014). The purpose of his study is to hopefully come up with a solution to ciliopathic diseases and a way to treat those who are effected by them.

Before seeing this article, I did not know this type of disease existed. Again, the cilia is such a small part of a cell. Initially, I knew it functioned in the interaction between the cell and its environment as well as some cases for movement. Never did I think that it would be the cause of many diseases that I know today.


For more information on the topic, the Ciliopathy Alliance has an extensive website with much information on the subject and can be accessed here.


References

Waters, A., & Beales, P. (2011, January 6). Ciliopathies: An expanding disease spectrum. Retrieved February 27, 2015, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098370/

Welcome to the Ciliopathy Alliance. (2014, January 1). Retrieved February 27, 2015, from http://www.ciliopathyalliance.org/



Friday, February 20, 2015

Encounter: Journal Post 6

This week in lab, we discovered who our culprit was for our DNA fingerprinting experiment. After being able to partake in such an experiment that allows someone to determine whether someone was at the scene of the crime or not was pretty exciting. The knowledge that can be found from a single cell sample is remarkable.
At home I was watching TV when I came across an episode of CSI and was captured by the context of the episode. They off course were investigating a murder and were finding ways to determine who the killer was. Afterward, I began watching a show with my mom on Investigation Discovery and again, they used the same method to determine who the murder was.
I then began to think how spectacular the science community is and how it has grown. So much can be determined by a single cell. Someone accidentally leaves a hair somewhere that a murder occurs and they are immediately a suspect.
While things like this probably don't cross people's minds on a regular basis, being exposed to the methods of DNA Fingerprinting made things like this more evident.

Friday, February 13, 2015

Dictionary: Journal Post 5


This is week in class focused on COP I, COP II, and Clathrin. These are types of vesicle coat proteins which transport proteins from the rough Endoplasmic Reticulum to the Golgi apparatus.

COP I-
This protein transports from the Golgi to the ER.
This consists of ARF-1 and the coat protein present is coatomer. These components polymerize into a cage like structure which breaks down the membrane to a bud.

COP II-
This protein works opposite the way COP I works. It moves from the ER to the Golgi.
This consists of  protein dimers Sar-1 , Sec 23/24, and Sec 13/31 as coat proteins.

Clathrin-
This protein transport to various places from the Golgi. Pathways include from the Golgi to lysosomes, Golgi to endosomes, and Golgi to plasma membranes.
Coat proteins for include, ARF-1 and Clathrin.

The following images help illustrate the paths each protein takes as well as their structures.

 

For more useful information about how vesicle proteins work and helpful animations, click here.

Friday, February 6, 2015

Reflection: Journal Post 4

The last few weeks in lab have been focusing on PCR and on discovering if certain products were genetically modified. Such an investigation caused me to become more interested in the food around me. I went grocery shopping the other day at my local Trader Joes and Costco. It hadn't really occurred to me to check if some foods were GMO until this investigation. I read through many of the products I purchased at Trader Joe's and was pleased with the amount of organic foods. I then ventured to Costco and went on the same journey. I was pleasantly surprised with how many of the products they have were not genetically modified although the majority were. It was just a different experience to realize how much genetics are important and how they can affect us everyday.
Another realization I had is how much I love experimentation. Of course I should. I am a science major. But I have such a love for learning new things and finding out the truth for myself. It is so rewarding to know something because you yourself took the steps and time to make the discovery.
I enjoyed the lab conducted these past few weeks and look forward to the weeks coming.

Thursday, January 29, 2015

Encounter: Journal Post 3

My cell phone is a major part of my life. I use it to communicate with people, to keep myself entertained, to keep in touch with the world, and for many other purposes. When I have nothing to do, you can often catch me surfing the web and doing other trivial things on it. Today, as I waited for my next class to start, I was doing just that. I was surfing the web, going through pages and pages of useless information. I caught myself on NY Daily News and came across a very interesting news article entitled "Breakthrough 'Artificial Pancreas' Helps Control Diabetic's Insulin Levels." After reading that, I thought to myself--as most Cell Bio students would--what a great topic for my blog.

Now the story isn't as remarkable as it sounds, but it's still pretty fascinating. The article spoke of an app that was developed for study which gives insulin users a piece of mind. The purpose of the app is to calculate the insulin that's needed for each dose through the use of a sensor. The user wears the sensor which helps calculate the glucose levels in the body. This information is sent to the smartphone which then relays through a bluetooth connection to a pump. The pump then administers the correct dose of insulin, adjusting it as needed throughout the day. Thanks to this medical breakthrough, the app is now being dubbed the "artificial pancreas."

The app was developed by a team who is researching ways to make insulin administration easier, paying particular attention to those affected by Type 1 Diabetes. Dr. Carol Levy, lead investigator, and her team conducted a five-night sleep study on ten patients. The blood sugar levels were studied over night, allowing the team to view when the levels were low and how the app reacted to this scenario. The app was very particular and responded to even the smallest shifts. Overall, they felt it was a successful study but continue to fine-tune the app.

The app is still a few years away from being available due to FDA approval but it looks very promising. While Type 1 diabetics are the focus of the group, the team hopes to make the app available for Type 2 diabetics as well.

This encounter relates to the class in very obvious ways. Diabetes is a disease very much affected by the way cells work together. It can be characterized as a metabolic disease that prevents insulin production or prevents the body from reacting to insulin appropriately.

This encounter brought to my attention that we are becoming much more advanced at a more rapid rate. It's great that people are trying to simplify the way we live, especially when it deals with something as important as diabetes. This medical breakthrough is something that intrigued me very much. As I stated earlier, my phone is practically an extension of myself. Many people today would agree with that statement themselves. Such an innovation is paving the way for more advancements in medicine and technology. 

Resources: 

"Breakthrough 'Artificial Pancreas' Helps Control Diabetic’s Insulin Levels" article

Thursday, January 22, 2015

Reflection: Journal Post 2

This week in class, we learned about different cellular processes including metabolism and simple functions of the cell. As we went deeper into discussion, a very powerful thought crossed my mind. I marveled at the copious amounts of work these cells endure. Each plays an important role in the overall makeup of the body. Such little details make a world of difference. Then, it hit me. All these things, these tiny little details, have the same Creator. This is a Being so powerful and meticulous that He cared enough to create a purpose for each cell within us. Just as He created a purpose for everything on this earth. 

I admit I am a little nervous about this class because there are so many details to remember. So many things that I knew once and had forgotten are starting to pop up again. It makes me wonder if I'll be able to keep up. As I think of this, I also keep in mind that I have a great purpose in this world. Whether I struggle or not, I know that success is in my future. So long as I focus and keep my eyes on the prize, I am able to succeed. 

Every science class I've taken at this university has made me come to this realization. I know that my thoughts and aspects would be different had I gone to another university. But again, it's the tiny details that mattered. Its the tiny details that make me who I am.



Thursday, January 15, 2015

Dictionary: Journal Post 1

There were a few terms that were meant as review which were mentioned in class this week.

While these terms are familiar, I felt it was difficult to imagine a definition for the term.

These terms include:





Nuceloside - a molecule which consists of a nitrogenous base linked to a sugar but lacks a phosphate group. This is different from a nucleotide which contains a phosphate group.






Cell Fractionation - This method allows researched to separate a cell in order to isolate the part of study. This method is often used in the study of organelles and other studies regarding the mechanics of a cell.
The following link provides a very detailed animation, providing insight on the process of cell fractionation.



Alpha Carbohydrates - α-carbohydrates are characterized by a cis configuration, meaning the OH group and CH2OH group are on opposite sides of the ring. This is also characterized by the OH group pointing downward.

Beta Carbohydrates - β-carbohydrates are characterized by trans configuration, meaning the OH group and CH2OH group are on the same side of the ring. The OH group is pointing upward.